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Wednesday, June 18, 2014

Cheyeanne Perez summary

Yesterday was full of very exciting things besides all the reading. the first thing i did yesterday was read a packet about DNA based Information Technologies. I learned all the new words and processes DNA  takes to replicate. Then i read a chapter in a book about the protocols and steps to preform a transformation. I began to preform transformations of plasmids into bacteria cells. we began by getting all of the bacteria cells as well as the plasmids we needed and kept them on ice. Then we labeled all of the tubes used to mix the cells. Then we measured out all of the plasmids and bacteria cells and placed them in their designated tubes. Then we allowed the tubes to sit in the ice bath for thirty minutes then heat shocked them in forty seven degree Celsius water for forty five seconds to have the plasmids enter the bacteria cells. then We added the right amount of nutrients to each tube then placed the cells onto petri dishes to incubate in thirty seven degree Celsius  over the night to show growth in the morning.


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