The part of my research that I had to do today was collecting a colony from one of my multiple sample plates that grew over the night last night. Each colony that I collected was done by using sterilized pipet tips to simply lift the colony off of the petri dish and then placed into falcon tubes that contained nutrients as well as the antibiotic ampicillin. Ampicillin is used to only allow certain cells to grow that contain the specific plasmid that was introduced by us, other DNA and cells will not reproduce and clone because they are not resistant to ampicillin. Then after all the sample colonies were removed from the plates and the tips were placed into the tubes, the tubes are then placed into the shake incubator overnight to clone each of the colonies DNA and cells.
Tomorrow I will remove the tubes from the shake incubator and preform a mini prep on the newly cloned DNA to remove the cells and other unwanted parts. Then after all that needs to be removed is discarded the DNA will be all that is left in a simple saline solution. This will then be measured and read to test if the mutation was added correctly.
4 days down:36 more left to make memories
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